Medium1 markStructured
Infection and ResponseHigherquantitative analysismicrobiologyrequired practical

AQA GCSE · Question 03.7 · Infection and Response

Figure 4BACDEPaper discscontainingantibioticPaper discwith noantibioticSalmonellabacteria growing

The scientists wanted to be more certain about which antibiotic should be prescribed. Describe how the results in Figure 4 could be used to obtain a quantitative comparison of antibiotics A and B.

How to approach this question

The word "quantitative" means getting a numerical result. How could you measure the clear zones for A and B to get numbers that you can compare? Think about the geometry of the zones.

Full Answer

Measure the diameter or radius of the zones of inhibition for A and B and calculate their areas (using πr²). The one with the larger area is more effective.
A visual comparison can be subjective. To get a quantitative (numerical) comparison, the scientists should measure the zones of inhibition. The most accurate method is to: 1. Use a ruler to measure the diameter of the clear zone for both antibiotic A and antibiotic B. 2. Calculate the radius (diameter / 2) for each. 3. Calculate the area of each circular zone using the formula: Area = πr². The antibiotic that corresponds to the larger calculated area is the more effective one. Simply comparing diameters is also a valid quantitative comparison, but calculating the area is a more complete method.

Common mistakes

✗ Just saying "measure the zones" without specifying what to measure (diameter, radius, or area). ✗ Forgetting to mention calculating the area, as this is the best measure of the total effect.

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